open positions

postdoctoral associate

The Briney lab is looking for a highly motivated postdoctoral associate to lead our systems biology efforts as part of the newly funded Center for Viral Systems Biology (CViSB). The mission of the CViSB is to is to identify molecular networks of host and viral factors that determine the outcome of infection with Ebola (EBOV) and Lassa (LASV) viruses. As a leader of the CViSB’s Technology Core, this position will involve generating and analyzing next-generation sequencing data from antibody and TCR repertoires, implementation and utilization of high-throughput single-cell techniques (DropSeq, SplitSeq, 10X Genomics, etc), and development of novel computation tools for analysis and integration of large bulk and single-cell sequencing datasets. Applicants should have a strong publication record and demonstrated expertise in generating and processing large-scale immune receptor data. Programming experience is not strictly required but is highly desirable.

responsibilities
  • Create and improve techniques for large-scale, cost efficient sequencing of adaptive immune receptor repertoires (antibody and TCR)
  • Oversee and directly participate in the isolation and characterization of neutralizing monoclonal antibodies from survivors of Lassa and Ebola infection
  • Work closely with computational biologists to develop novel computational tools for evaluation of antibody repertoire development, maturation and function
  • Train and supervise junior scientists and students
qualifications
  • Ph.D. in immunology, genetics, computational biology, or similar is required
  • Independent, highly motivated and passionate about harnessing emerging technology to answer fundamental questions about viral immunology
  • Familiarity with common molecular biology techniques (cloning, protein production and purification, assessment of antibody binding/neutralization) is highly desirable
  • Experience performing next-generation sequencing is highly desirable, especially experience with the 10X Genomics Chromium platform or other microfluidics-based single cell analysis platforms
  • Experience handing and manipulating large sequence datasets is highly desirable
  • Programming skills in a scripting language (our lab primarily uses Python) are not required but would be a major plus. Although programming proficiency is not required, the ability to assist in the development of novel computational tools by providing immunological guidance and support to computationally-focused lab members is critical
  • Strong interpersonal skills and the ability to work with and mentor junior scientists and students

To apply, please email your CV and a cover letter to briney@scripps.edu.

research technician

The Briney lab is looking for a highly motivated research technician to join our systems biology efforts as part of the newly funded Center for Viral Systems Biology (CViSB). The mission of the CViSB is to is to identify molecular networks of host and viral factors that determine the outcome of infection with Ebola (EBOV) and Lassa (LASV) viruses. As a leader of the CViSB’s Technology Core, this position will involve generating adaptive immune receptor repertoire (AIRR) next-generation sequencing libraries, utilization of high-throughput single-cell techniques (DropSeq, SplitSeq, 10X Genomics, etc), and functional evaluation of novel monoclonal antibodies using binding and/or neutralization assays. Previous molecular biology experience (PCR, cloning, ELISA, protein expression and purification, etc) is not strictly required but is highly desirable.

responsibilities
  • Isolation and cryopreservation of peripheral blood mononuclear cells from whole blood or leukapheresis samples
  • Library preparation for next-generation sequencing of adaptive immune receptor repertoires (antibody and TCR)
  • Participate in the isolation and characterization of neutralizing monoclonal antibodies from survivors of Lassa and Ebola infection
  • Work closely and efficiently with other scientists and students
qualifications
  • Bachelor’s degree in biology, immunology, genetics, or similar is required
  • Independent, highly motivated and passionate about harnessing emerging technology to answer fundamental questions about viral immunology
  • Familiarity with common molecular biology techniques (cloning, protein production and purification, assessment of antibody binding/neutralization) is highly desirable
  • Experience performing next-generation sequencing is highly desirable, especially experience with the 10X Genomics Chromium platform or other microfluidics-based single cell analysis platforms
  • Strong interpersonal and communication skills

To apply, please email your CV and a cover letter to briney@scripps.edu.